A Retrospective Evaluation of Topical Ozone and In- Socket PRF Application on Postoperative Pain, Edema, Trismus, Wound Healing and Mucosal Blood Stream After Third Molar Surgery

Authors

DOI:

https://doi.org/10.58600/eurjther3076

Keywords:

edema, mucosal blood flow, ozone therapy, pain, platelet rich fibrin, trismus, third molar tooth, wound healing

Abstract

Objective: The aim of this sudy is to evaluate the effects of topical ozone and in-socket platelet rich fibrin (PRF) applications on postoperative pain, edema, trismus and mucosal blood flow after third molar surgery retrospectively.

Methods: The data of 120 people with an impacted third molar tooth in a position of Class II and devision B to Pell Gregory classification having surgery in Oral and Maxillofacial Surgery Clinics of Gaziantep University, Faculty of Dentistry throughout a two years period were collected. They were offered to have postoperative procedures. Topical ozone and in-socket PRF applications were determined as two alternatives. Besides these two options the ones who did not like to receive any of them were surgically treated in the same manner with the others. All data were recorded independent from their choice and finally divided into three groups -with a randomized distribution within each- due to the after surgery procedures. The groups were determined as the first group having topical ozone (n=40) (F=31, M=9, mean age= ± 21.83), the second group having in-socket fibrin (n=40) (F=29, M=11, mean age= ± 22.68), and the third one having no additive therapy as a control group (n=40) (F=28, M=12, mean age= ± 23.58). All patients having the routine surgery protocols also were asked to answer a visual analog scale to score the postoperative pain. Evaluations included; the edema by lineer measurements on face, trismus by maximal mouth opening, mucosal blood flow by Laser Doppler Flowmeter, both preoperatively, and on days three and seven postoperatively. Also wound healing was evaluated by LTH score.

Results: On both postoperative in-socket PRF and topical ozone groups the clinical values were statistically better than the control group (p<0.050). While the ozone applied group exhibited better results in terms of pain and trismus on early period of healing period, the in-socket PRF applied group exhibited significant advantage on wound healing, edema and blood flow (p<0.050).

Conclusion: Topical ozone and in-socket PRF procedures are effective in supporting postoperative recovery of third molar surgery. Effects of combined application of these useful techniques may further find place in scientific researches as a new topic.

References

[1] Hupp J, Tucker M, Ellis E (2013) Contemporary Oral and Maxillofacial Surgery, 6th edn. Elseiver, St Louis.

[2] Gözlüklü Ö, Ulu M, Gözlüklü HÖ, Yilmaz N (2020) Comparison of Different Kinesio Taping Techniques After Third Molar Surgery. J Oral and Maxillofac Surg. 78(5):695–704. https://doi.org/10.1016/J.JOMS.2019.12.026

[3] Apers S, Luyckx K, Moons P (2013) Is quality of life the ultimate outcome parameter? Eur J Cardiovasc Nurs. 12(6):502–504. https://doi.org/10.1177/1474515113512735

[4] Isola G, Matarese M, Ramaglia L, Cicciù M, Matarese G (2019) Evaluation of the efficacy of celecoxib and ibuprofen on postoperative pain, swelling, and mouth opening after surgical removal of impacted third molars: a randomized, controlled clinical trial. Int J Oral Maxillofac Surg. 48(10):1348–1354. https://doi.org/10.1016/J.IJOM.2019.02.006

[5] Alcântara CEP, Falci SGM, Oliveira-Ferreira F, Santos CRR, Pinheiro MLP (2014) Pre-emptive effect of dexamethasone and methylprednisolone on pain, swelling, and trismus after third molar surgery: a split-mouth randomized triple-blind clinical trial. Int J Oral Maxillofac Surg. 43(1):93–98. https://doi.org/10.1016/J.IJOM.2013.05.016

[6] Chaves ME de A, Piancastelli ACC, de Araújo AR, Pinotti M (2014) Effects of low-power light therapy on wound healing: LASER x LED. An Bras Dermatol. 89(4):616. https://doi.org/10.1590/ABD1806-4841.20142519

[7] Emshoff R, Moschen I, Strobl H (2004) Use of laser Doppler flowmetry to predict vitality of luxated or avulsed permanent teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 98(6):750–755. https://doi.org/10.1016/j.tripleo.2004.05.003

[8] Bocci V (2011) Ozone: A new medical drug, 2nd edn. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9234-2

[9] Nogales CG, Ferrari PH, Kantorovich EO, Lage-Marques JL (2008) Ozone therapy in medicine and dentistry. J Contemp Dent Pract. 9(4):75–84.

[10] Sivalingam VP, Panneerselvam E, Raja KVB, Gopi G (2017) Does Topical Ozone Therapy Improve Patient Comfort After Surgical Removal of Impacted Mandibular Third Molar? A Randomized Controlled Trial. J Oral Maxillofac Surg. 1;75(1):51.e1-51.e9. https://doi.org/10.1016/J.JOMS.2016.09.014

[11] Kazancioglu HO, Ezirganli S, Demirtas N (2014) Comparison of the influence of ozone and laser therapies on pain, swelling, and trismus following impacted third-molar surgery. Lasers Med Sci. 29(4):1313–1319. https://doi.org/10.1007/S10103-013-1300-Y

[12] Azarpazhooh A, Limeback H (2008) The application of ozone in dentistry: a systematic review of literature. J Dent. 36(2):104–116. https://doi.org/10.1016/J.JDENT.2007.11.008

[13] Patel PV, Kumar V, Kumar S, Vidya GD, Patel A (2011) Therapeutic effect of topical ozonated oil on the epithelial healing of palatal wound sites: a planimetrical and cytological study. J Investig Clin Dent. 2(4):248–258. https://doi.org/10.1111/J.2041-1626.2011.00072.X

[14] Maglia DR, Souza B do AF, Visioli F (2024) Efficacy of ozone therapy for oral mucosa wound healing: a systematic review and meta-analysis. Clin Oral Investig. 28(9):490. https://doi.org/10.1007/S00784-024-05873-2

[15] Filippi A (2000) Klinisch-experimentelle Untersuchungen zur Wundheilung von Oralgeweben unter Einfluß von Ozonwasser. Fachverl, Köhler.

[16] Akdeniz SS, Beyler E, Korkmaz Y, Yurtcu E, Ates U, Araz K, Sahin FI, Torun OY (2018) The effects of ozone application on genotoxic damage and wound healing in bisphosphonate-applied human gingival fibroblast cells. Clin Oral Investig. 22(2):867–873. https://doi.org/10.1007/S00784-017-2163-6

[17] Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJJ, Mouhyi J, Gogly B (2006) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 101(3). https://doi.org/10.1016/J.TRIPLEO.2005.07.009

[18] Dohan Ehrenfest DM, Rasmusson L, Albrektsson T (2009) Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF). Trends Biotechnol. 27(3):158–167. https://doi.org/10.1016/J.TIBTECH.2008.11.009

[19] Nurden AT (2011) Platelets, inflammation and tissue regeneration. Thromb Haemost. 105(SUPPL. 1):13–33. https://doi.org/10.1160/THS10-11-0720

[20] Del Fabbro M, Bucchi C, Lolato A, Corbella S, Testori T, Taschieri S (2017) Healing of Postextraction Sockets Preserved With Autologous Platelet Concentrates. A Systematic Review and Meta-Analysis. J Oral Maxillofac Surg. 75(8):1601–1615. https://doi.org/10.1016/J.JOMS.2017.02.009

[21] Gülşen U, Şentürk MF (2017) Effect of platelet rich fibrin on edema and pain following third molar surgery: a split mouth control study. BMC Oral Health. 17(1). https://doi.org/10.1186/S12903-017-0371-8

[22] Salas GA, Lai SA, Verdugo-Paiva F, Requena RA (2022) Platelet-Rich Fibrin in Third Molar Surgery: Systematic Review and Meta-Analysis Protocol. Craniomaxillofac Trauma Reconstr. 15(2):164–168. https://doi.org/10.1177/19433875211016203

[23] Choukroun J, Diss A, Simonpieri A, Girard MO, Schoeffler C, Dohan SL, Dohan AJJ, Mouhyi J, Dohan MD (2006) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part V: histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 101(3):299–303. https://doi.org/10.1016/J.TRIPLEO.2005.07.012

[24] Anitua E, Andia I, Ardanza B, Nurden P, Nurden AT (2004) Autologous platelets as a source of proteins for healing and tissue regeneration. Thromb Haemost. 91(1):4–15. https://doi.org/10.1160/TH03-07-0440

[25] Clark RAF (2001) Fibrin and wound healing. Ann N Y Acad Sci. 936:355–367. https://doi.org/10.1111/J.1749-6632.2001.TB03522.X

[26] Broos K, Feys HB, De Meyer SF, Vanhoorelbeke K, Deckmyn H (2011) Platelets at work in primary hemostasis. Blood Rev. 25(4):155–167. https://doi.org/10.1016/J.BLRE.2011.03.002

[27] Dragonas P, Katsaros T, Avila-Ortiz G, Chambrone L, Schiavo JH, Palaiologou A (2019) Effects of leukocyte-platelet-rich fibrin (L-PRF) in different intraoral bone grafting procedures: a systematic review. Int J Oral Maxillofac Surg. 48(2):250–262. https://doi.org/10.1016/J.IJOM.2018.06.003

[28] Mozzati M, Gallesio G, Tumedei M, Del Fabbro M (2020) Concentrated Growth Factors vs. Leukocyte-and-Platelet-Rich Fibrin for Enhancing Postextraction Socket Healing. A Longitudinal Comparative Study. Appl Sci. 10(22):8256. https://doi.org/10.3390/APP10228256

[29] Kumar N, Prasad K, Ramanujam L, Ranganath R, Dexith J, Chauhan A (2015) Evaluation of treatment outcome after impacted mandibular third molar surgery with the use of autologous platelet-rich fibrin: a randomized controlled clinical study. J Oral Maxillofac Surg. 73(6):1042–1049. https://doi.org/10.1016/J.JOMS.2014.11.013

[30] Sybil D, Sawai M, Faisal M, Singh S, Jain V (2020) Platelet-rich fibrin for hard- and soft-tissue healing in mandibular third molar extraction socket. Ann Maxillofac Surg. 10(1):102–107. https://doi.org/10.4103/AMS.AMS_228_19

[31] Matthews B, Vongsavan N (1993) Advantages and limitations of laser Doppler flow meters. Int Endod J. 26(1):9. https://doi.org/10.1111/J.1365-2591.1993.TB00531.X

Figure 1. The measurement points A, B and C on oral mucosa.

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Published

2026-06-12

How to Cite

Karslı, E. D., & Kart, İlker. (2026). A Retrospective Evaluation of Topical Ozone and In- Socket PRF Application on Postoperative Pain, Edema, Trismus, Wound Healing and Mucosal Blood Stream After Third Molar Surgery. European Journal of Therapeutics. https://doi.org/10.58600/eurjther3076

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